ICSI and IMSI are techniques used in cases of male factor infertility, allowing male patients with low sperm count, poor sperm motility and/or abnormal sperm structure the chance to father a child
ICSI – INTRACYTOPLASMIC SPERM INJECTION
ICSI can be useful in cases of low sperm count, low motility, abnormal morphology and presence of high levels of antibodies in the semen, as well as in cases of a previously performed vasectomy. It is the most common technique used to treat male factor infertility.
Following preparation (which is the same as for a standard IVF treatment, including stimulation and oocyte retrieval), a single sperm with normal morphological features is identified (using a 400x microscope), and injected into the center (cytoplasm) of a mature oocyte (held with a pipette) through a specialized needle. The following morning the oocyte is tested to see if the procedure resulted in an embryo being created. The created embryos are treated in the same manner as during standard IVF treatments.
MSOME – Motile Sperm Organelle Morphology Examination
MSOME is a procedure which allows the examination of a motile spermatozoon under a powerful microscope, which makes it possible to spot defects which were not detectable under lesser optical magnification, thus ensuring that only the most high-quality spermatozoa are used for egg fertilization. Among the organelles assessed are: the acrosome, postacrosomal lamina, neck, tail, mitochondria and nucleus. Special attention is paid to the nucleus, which is assessed in terms of shape and content (vacuolar area). At Atlas Fertility MSOME is performed under magnification of 6300x.
IMSI- Intracytoplasmic Morphologically Selected Sperm Injection
The development of MSOME facilitated the introduction of IMSI – a microinjection procedure, which is a modification of the standard ICSI method. It is advised for patients with recurrent IVF failures and in cases where high numbers of DNA-fragmented spermatozoa had been detected, and has been found effective in assisting said patients to conceive. It should, however, be noted that as the process is more complex than ICSI, it is also more time consuming and costly.