Gametes can undergo cryopreservation for long term storage. Samples are kept in Liquid Nitrogen (-196ºC) and stored in specialized liquid nitrogen dewars. The dewars are screened for HIV and Hepatitis viruses as required prior to cryopreservation of samples.
A procedure to preserve sperm cells. Sample is cryopreserved in specialized cryovials and can be successfully used for the desired treatment.
The main reasons for cryopreserving semen samples are:
- After semen surgical collection (MESA, TESE, TESA).
- In case of a low sperm count, running a risk of becoming total azoospermic / suffer further compromise of sperm quality.
- As a means of guarantee & aid in Overcoming a psychological barrier to give a sperm sample on the day of egg collection. The existence of frozen sperm gives them the added security that their sperm will be available when needed for the treatment.
- As a means to cope with future azospermia.
- non-simultaneous availability of the sample- in case of geographical or temporal distance between donors and recipients.
- As means of a lifetime guarantee.
Oocytes are cryopreserved using a procedure called “Vitrification”. It is a rapid and simple cryopreservation method which is based on introducing solidification of the cells and the surrounding vitrification solution, thus preventing formation of ice crystallization in the intra-cellular and extra-cellular space.
The main reasons for cryopreserving oocytes are the following:
- Should your body experience stimulation with hormonal medication resulting in the production of several follicles and the retrieval of multiple oocytes; which can exceed the optimal number of oocytes (surplus oocytes). The remaining of your ooctyes can then be cryopresevered for future use, if the embryo cryopreservation can no longer remain a feasible option for you.
- Stimulation with hormonal medication results in the production of several follicles and the retrieval of multiple oocytes. When the optimal number of oocytes is exceeded, the “surplus oocytes” can be cryopreserved for use at a later time in the future when embryo cryopreservation is not feasible.
- Fertility preservation for patients without a male partner.
- Young women who wish to delay motherhood.
- Females with time constraints or contraindications to ovarian stimulation.
- Conditions that may lead to wastage of their reproductive potential.
- Minimization of Ovarian Hyper stimulation Syndrome risk.
- Oocyte accumulation in low responder patients.